Genesis Protein

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Genesis, Eduardo Kac, 1998/99. This diagram shows the plasmid (left) and the gene used in the transgenic artwork "Genesis". A plasmid is an extrachromosomal ring of DNA. The black circular arrow at the top of the plasmid indicates the direction of transcription (i.e., the process by which one strand of DNA is copied into a single strand of RNA). Shown in the illustration are: 1) Promoter sequence (sequence of DNA where RNA polymerase binds on to begin transcription); 2) Multiple Cloning Site (part of the plasmid that has been engineered to accept the insertion of other sequences); 3) Cyan fluorescence sequence (sequence of DNA that codes for cyan fluorescent protein); 4) MCS with GENESIS gene (site where the Genesis gene was inserted); 5) Ampicilin resistance sequence (gene that codes for resistance to the antibiotic ampicilin); 6) Origin of replication site (site where the process of replication of DNA molecules originates, by one single strand being used as a template for the production of another single strand). This plasmid, with the Genesis gene, was incorporated into E. coli bacteria. Genesis bacteria share a Petri dish with another colony of E. coli bacteria that have yellow fluorescence but which do not have the Genesis gene.